Cyk3.jpg

SH3-domain protein located in the bud neck and cytokinetic actin ring; relocalizes from bud neck to nucleus upon DNA replication stress; activates the chitin synthase activity of Chs2p during cytokinesis; suppressor of growth and cytokinesis defects of chs2 phospho-mutants 1 2 3 4. However, we consider this to be unlikely because the phenotype of cyk3Δ is more severe than cyk3 TGcΔ, and unlike cyk3 TGcΔ, mutations in the N-terminal region (cyk3 W45A and cyk3 P188,191A) did not show genetic interactions with sac7Δ. Both of these results suggest that the N-terminal and C-terminal regions of Cyk3 have genetically. A role for Cyk3 in primary septum formation is also supported by the finding that hyperactive Chs2 genetically rescues defects associated with a Cyk3 deletion [79]. Whether Cyk3 and Inn1 have a direct function in regulating AMR contractility is not known. Hof1 was shown to bind to the AMR through a ring localization sequence (RLS) [85]. In Saccharomyces cerevisiae, it is well established that Hof1, Cyk3, and Inn1 contribute to septum formation and cytokinesis. Because hof1∆ and cyk3∆ single mutants have relatively mild defects but hof1∆ cyk3∆ double mutants are nearly dead, it has been hypothesized that these proteins contribute to parallel pathways. However, there is also evidence that they interact physically. Cytokinesis requires the wholesale reorganization of the cytoskeleton and secretion to complete the division of one cell into two.

Fission yeast Cyk3p is a transglutaminase-like protein that - In the budding yeast Saccharomyces cerevisiae, the IQGAP-related protein Iqg1 (Cyk1) promotes cytokinetic actin ring formation and is required for cytokinesis and viability [1-3]. As the actin ring is not essential for cytokinesis or viability, Iqg1 must act by. Cells were grown on medium containing 0 or 70 µM methionine to fully or modestly induce the expression of the fusion genes. (E) Anti-MYC immunoblot of extracts originating from cells expressing MYC-labeled CYK3 (lane 2), a mutant of CYK3 lacking the proline-rich stretch between residues 183 and 191 (lane 3) or the empty PRS315 plasmid (lane 1). A cyk3 null strain was constructed and its phenotype analyzed. The cyk3 null mutant was viable although growth was slowed (data not shown). The cyk3 cells were 9-10% deficient in cytokinesis (see Supplementary material) and were moderately aberrant in morphology (Figure 2a,b); 50% of cyk3 cells exhibited a noticeably aberrant mother-bud neck, which often appeared large, bent, or misshapen. CYK3 suppressed the requirement for IQG1 in viability and cytokinesis without restoration of the actin ring, demonstrating that CYK3 promotes cytokinesis through an actomyosin-ring-independent pathway. CYK3 encodes a novel SH3-domain protein that was found in association with the actin ring and the mother-bud neck. cyk3 null cells had misshapen. Protein sequence for the given gene in S288C and other strains, when available. Use the pull-down menu under "Strain" to select the sequence for a specific strain. The displayed sequence can be downloaded in FASTA format as a . Amino acids displayed in blue represent modification sites. Cyk3 was originally identified as a dosage suppressor of iqg1 (Korinek et al.

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The kinetic landscape of cytokinesis. As the cell cycle progresses from the onset of mitotic exit to cell separation, distinct functional modules accumulate at the division site in a strict temporal order to play their specific roles in cytokinesis. The kinetics were modified from Figures 1, 2, 3, and 6. The most comprehensive image search on the web. Online image converter to JPEG. Convert your image to JPG from a variety of formats including PDF. Upload your files to convert and optionally apply effects. If you need more advanced features like visual cropping, resizing or applying filters, you can use this free online image editor. Cell morphogenesis is a complex process that relies on a diverse array of proteins and pathways. We have identified a transglutaminase-like protein (Cyk3p) that functions in fission yeast morphogenesis. The phenotype of a cyk3 knockout strain indicates a primary role for Cyk3p in cytokinesis. Correspondingly, Cyk3p localizes both to the actomyosin contractile ring and the division septum.